Journal: Microorganisms

Article Title: Development of Polymerase Chain Reaction–High-Resolution Melt Assay for Waterborne Pathogens Legionella pneumophila , Vibrio parahaemolyticus , and Camplobacter jejuni

doi: 10.3390/microorganisms12071366

Figure Lengend Snippet: Bacterial DNA utilized for specificity testing.

Article Snippet: Campylobacter jejuni , subsp. Jejuni , ATCC (33560D-5).

Techniques:

Journal: Antimicrobial Agents and Chemotherapy

Article Title: Cloning and Expression of Novel Aminoglycoside Phosphotransferase Genes from Campylobacter and Their Role in the Resistance to Six Aminoglycosides

doi: 10.1128/AAC.01682-17

Figure Lengend Snippet: Campylobacter strains used in this study

Article Snippet: The MIC was recorded as the lowest concentration of antimicrobial agent that completely inhibited the growth of an organism after incubation at 35°C for 16 to 20 h, according to guidelines of the Clinical and Laboratory Standards Institute (CLSI) ( 6 ) For conjugation, nine Gen r Campylobacter strains that carried variants of aph(2′′) genes were used as donor strains, including four C. jejuni (41912, 41921, 41902, and 41905) and five C. coli (41945, 41898, 41904, {"type":"entrez-nucleotide","attrs":{"text":"N29710","term_id":"1148230","term_text":"N29710"}} N29710 , and {"type":"entrez-nucleotide","attrs":{"text":"N20344","term_id":"1125299","term_text":"N20344"}} N20344 ) strains.

Techniques: Mutagenesis, Cloning

Journal: Poultry Science

Article Title: Molecular characterization of Campylobacter spp . isolates obtained from commercial broilers and native chickens in Southern Thailand using whole genome sequencing

doi: 10.1016/j.psj.2024.103485

Figure Lengend Snippet: Genome characteristics, sequence types, clonal complex and accession numbers of Campylobacter strains.

Article Snippet: The reference strain used for alignment and single nucleotide polymorphism ( SNP ) identification was Campylobacter jejuni strain (GenBank accession number AL111168.1) obtained from the National Center for Biotechnology Information ( NCBI ).

Techniques: Sequencing

Journal: Poultry Science

Article Title: Molecular characterization of Campylobacter spp . isolates obtained from commercial broilers and native chickens in Southern Thailand using whole genome sequencing

doi: 10.1016/j.psj.2024.103485

Figure Lengend Snippet: The relationship of ST of C.jejuni isolated from chickens with other ST reported in Thailand. (A): Venn-diagram shows the shared number of ST among different sources of C. jejuni isolates in this study with previously published studies in Thailand. (B): The minimum spanning tree illustrates the C. jejuni isolates isolated from chickens in this study with humans and chickens in previous studies in Thailand. The node numbers are the C. jejuni ST, and the different size of each node is the sum of the number of C. jejuni isolates. The distance of each pair of nodes is the number of discriminating alleles.

Article Snippet: The reference strain used for alignment and single nucleotide polymorphism ( SNP ) identification was Campylobacter jejuni strain (GenBank accession number AL111168.1) obtained from the National Center for Biotechnology Information ( NCBI ).

Techniques: Isolation

Journal: Poultry Science

Article Title: Molecular characterization of Campylobacter spp . isolates obtained from commercial broilers and native chickens in Southern Thailand using whole genome sequencing

doi: 10.1016/j.psj.2024.103485

Figure Lengend Snippet: The SNP-based phylogenetic tree of 21 C. jejuni isolates (A) and 5 C. coli isolates (B) with MLST (ST), AMR profile, ARG and mutations, and virulence traits in commercial broilers and native chickens. Red cells indicate phenotypic antimicrobial resistance, yellow cells indicate the presence of ARG/mutations, gray cells represent the absence of either phenotypic resistance or ARG/mutations, and white cells are not applicable for antimicrobial susceptibility testing. The gray bars show the total number of virulence-related genes in each Campylobacter isolate, and the dark gray bars illustrate the specific number of virulence factors associated with these genes for each Campylobacter isolate.

Article Snippet: The reference strain used for alignment and single nucleotide polymorphism ( SNP ) identification was Campylobacter jejuni strain (GenBank accession number AL111168.1) obtained from the National Center for Biotechnology Information ( NCBI ).

Techniques:

Journal: Poultry Science

Article Title: Molecular characterization of Campylobacter spp . isolates obtained from commercial broilers and native chickens in Southern Thailand using whole genome sequencing

doi: 10.1016/j.psj.2024.103485

Figure Lengend Snippet: Sankey plot illustrating association between hosts, Campylobacter spp., ST, phenotypic resistance, ARG and mutations. (*) The proportion of isolates exhibiting genotypic resistance (through ARG/mutations) within the total number of isolates showing phenotypic antimicrobials resistance.

Article Snippet: The reference strain used for alignment and single nucleotide polymorphism ( SNP ) identification was Campylobacter jejuni strain (GenBank accession number AL111168.1) obtained from the National Center for Biotechnology Information ( NCBI ).

Techniques:

Journal: Poultry Science

Article Title: Molecular characterization of Campylobacter spp . isolates obtained from commercial broilers and native chickens in Southern Thailand using whole genome sequencing

doi: 10.1016/j.psj.2024.103485

Figure Lengend Snippet: The circular SNP-based phylogenetic tree of 26 Campylobacter isolates of this study compared to 28 Campylobacter genomes originated in other Asian countries.

Article Snippet: The reference strain used for alignment and single nucleotide polymorphism ( SNP ) identification was Campylobacter jejuni strain (GenBank accession number AL111168.1) obtained from the National Center for Biotechnology Information ( NCBI ).

Techniques: